Safety

SDS Sheets: Available upon request on all chemicals used in this process.

PPE: The following should be worn by all lab personnel during Ethanol extraction:

  • Splash Goggle
  • Lab Coat
  • Gloves

Required Materials:

  • MagSil-PR®
  • n-Heptane
  • 99% USP Isopropyl Alcohol (optional for cleaning)

Preparation

  • Distilled cannabis oil will be mixed with n-Heptane, washed with pH adjusted saline, then passed over a MagSil-PR ® column.
    • Concentration – Use full concentration of:
      • n-Heptane
      • Magsil-PR®
    • Quantity –  2 parts n-Heptane: 1 part distilled cannabis oil: 1 part MagSil-PR ®
  • Frequency: An initial volume of distilled oil is used. An initial volume of n-Heptane is used, more Isopropyl Alcohol (optional) is used to flush the column for cleaning.
  • Location: Distillation of crude oil occurs in the short path still

Procedure

  1. Dissolve first pass distillate in n-Heptane (2:1, n-Heptane: Distillate)
  2. Add solution to reactor
  3. Prepare 60 parts saline (1-5% Salt to distilled water)
  4. Separate saline into 3 equal volumes
  5. Use Citric Acid to bring the pH of one volume of saline to pH 4.
  6. Use Sodium Bicarbonate to bring the pH of one volume of saline to pH8.
  7. Add pH 4 Saline to reactor
  8. Mix for 10 minutes
  9. Drain Saline
  10. Repeat Steps 7-9 with pH 8 saline
  11. Repeat Steps 7-9 with remaining neutral pH saline.
  12. Wet load MagSil-PR ® to Chromatography column, collect all fractions together.
  13. Drain solution from reactor to top of chromatography column, collect all fractions together.
  14. Before column runs dry, flush volume of clean n-Heptane through column
  15. Before column runs dry, swap collection vessel and flush volume of clean Isopropyl
    • Cleaning is highly recommended to prevent reintroduction of Pesticides between different batches and/or runs.
  16. Rotovap n-Heptane solution
  17. Second pass SPD